The invention provides a method for extracting tremella polysaccharides by enzyme process assisted wet pulping. The method comprises the following steps: crushing fresh tremella, and adding water; adding pectinase and cellulase, and carrying out enzymatic hydrolysis for 2-3 h; heating the obtained mixture to 70-90 DEG C, and pulping the heated mixture for 5-10 min; centrifuging the obtained solution at 1500-3000 rpm for 10-20 min, and collecting the obtained supernatant; adding trypsin to the supernatant, and carrying out enzymatic hydrolysis for 2-3 h; carrying out vacuum concentration on thesupernatant; adding an organic solvent to the obtained concentrate, standing the obtained solution at 20-30 DEG C for 1-2 h, and separating and retaining the obtained upper concentrate; dialyzing theconcentrate in distilled water by a nano-film; adding the organic solvent to the dialyzed concentrate, and filtering the obtained solution to completely volatilize the organic solvent in order to obtain a precipitate; and adding distilled water to the precipitate until the precipitate completely dissolved, and placing and freeze-drying the obtained solution in a freeze dryer to obtain the tremella polysaccharides. The method has the advantages of high yield of the prepared tremella polysaccharides, mild operation conditions of the enzyme process assisted wet pulping, high efficiency, energy saving and low pollution.