The present invention discloses a prawn bait additive containing a traditional Chinese medicine active probiotic culture medium. Micro-organisms used in a prawn bait are subjected to fermentation culture by using the traditional Chinese medicine active probiotic culture medium. The traditional Chinese medicine active probiotic culture medium comprises the following components: wheat bran, soybeanmeal, corn flour, brown sugar, traditional Chinese medicine components, light calcium carbonate, a yeast extractum, laminaria japonica and fulvic acid; and the traditional Chinese medicine componentscomprise radix astragali, radix pseudostellariae, white atractylodes rhizome, Chinese rhubarb, radix isatidis, radix scutellariae, wild chrysanthemum flowers, radix gentianae, rhizoma alismatis, spicaprunellae and decumbent bugle herb. The prawn bait additive solves problems that the micro-organisms in microecological bait additives for prawns in the prior art are few in the number, low in activity, weak in colonization ability in intestinal tracts, etc., thereby increasing the number of the micro-organisms in the microecological bait additives for the prawns, improving the activity of the micro-organisms and the colonization ability of the micro-organisms in the intestinal tracts.