The invention provides a preparation method of a low-adhesion wound dressing. The preparation method includes the steps of firstly, introducing 2-bromoisobutyl acyl bromide serving as a modifier to the surface of a cellulose material by the aid of a surface-initiated electron transfer activated regenerated catalyst atom transfer radical polymerization technique to prepare a cellulose-based macro-initiator; secondly, controllably building a gel-like molecular brush structure at the initiation locus of the surface of the cellulose material by N-isopropylacrylamide in a nitrogen environment, andperforming washing and drying to prepare the low-adhesion wound dressing. The prepared wound dressing has the advantages that gel molecules and the cellulose material are firmly combined in a covalentbond form to prepare the wound dressing, the wound dressing has excellent low-adhesion performance, secondary trauma caused by the adhesion between new granulation tissue and a traditional gauze dressing is avoided, a new idea is provided for the preparation of low-adhesion wound dressing, and the low-adhesion wound dressing is promising application prospect and market prospect.