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发明公开
CN109971874A 检测鸡毒支原体F弱毒疫苗株的引物对及方法 无效 - 驳回

检测鸡毒支原体F弱毒疫苗株的引物对及方法
摘要：
本发明公开了一种检测鸡毒支原体F弱毒疫苗株的引物对及方法，其中，该引物对包括：(上游)AATggATCCATgAAAATggAggTCA，(下游)ACTCTCgAgTTAAATgTAggTgAgAT；检测方法包括以下步骤：提取待测样品的DNA，以提取到的DNA为模板、以前述引物对为引物进行PCR扩增反应，PCR扩增反应结束后，对PCR扩增产物进行琼脂糖凝胶电泳。本发明的有益之处在于：本发明设计了对鸡毒支原体F弱毒疫苗株具有特异扩增作用的PCR引物对，并已经对鸡毒支原体TS11疫苗、鸡毒支原体F疫苗、鸡毒支原体6/85疫苗、野毒R株与野毒S6株为供试材料进行了验证，只有鸡毒支原体F弱毒疫苗株的样品能特异性地扩增出一条356bp的电泳条带，这说明本发明设计的引物对具有很强的特异性和准确性。
摘要（英）：

The invention discloses a primer pair and method for detecting mycoplasma gallisepticum F attenuated vaccine strains. The primer pair comprises AATggATCCATgAAAATggAggTCA upstream and ACTCTCgAgTTAAAtgTAggTgAgAT. The method comprises the following steps of extracting DNA (deoxyribonucleic acid) of a sample to be detected; taking the extracted DNA as the template to perform PCR (polymerase chain reaction) amplification with the primer pair, and after the PCR amplification is completed, performing agarose gel electrophoresis on PCR amplification products. The primer pair and method for detecting mycoplasma gallisepticum F attenuated vaccine strains has the advantage that the PCR primer pair with specific amplification effects on the mycoplasma gallisepticum F attenuated vaccine strains is designed, and through verification test with mycoplasma gallisepticum TS11 vaccines, mycoplasma gallisepticum F vaccines, mycoplasma gallisepticum 6/85 vaccines, wild R strains and wild S6 strains as testmaterials, only samples of the mycoplasma gallisepticum F attenuated vaccine strains are tested to be capable of specifically amplifying a 365 bp electrophoretic band, so that high specificity and accuracy of the designed primer pair can be proved.

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