The invention relates to a tissue culture method of lycoris incarnata, and belongs to the technical field of plant tissue culture and rapid propagation. The tissue culture method of the lycoris incarnata comprises the following steps of 1, inoculating sterilized lycoris incarnata bulbs into a cluster-bud induction culture medium for induction culture to obtain first bulblets; 2, inoculating the first bulblets into a callus induction culture medium for inducing callus culture to obtain callus lumps; 3, inoculating the callus lumps to a callus differential culture medium for differentiation culture to obtain second bulblets; 4, inoculating the second bulblets to a bud strengthening and rooting culture medium for bud strengthening and rooting culture. The culture method is high in safety andstrong in differentiation capacity, and efficient breeding of the lycoris incarnata can be achieved.