The invention belongs to the field of separation and preparation of natural functional substances, and relates to an extraction method of alkyl resorcinol in wheat bran. The method comprises the following steps: selecting dried wheat bran as a raw material, selecting, arranging and grinding the raw material, then adding ethyl acetate to carry out ultrasonic extraction, carrying out centrifuging toobtain a supernate, and carrying out rotary evaporation to obtain a concentrated solution; and then carrying out silica gel column chromatography by using a solid-phase extraction or a silica gel mixing sample for separation and purification, and further concentrating the eluent to obtain a mixed solution of different homolog of alkyl resorcinol. Through high performance liquid chromatography analysis, different homolog in the alkyl resorcinol product has the advantages of good separation degree, high purity and the like. Compared with the existing alkyl resorcinol preparation technology, themethod disclosed by the invention has the advantages that silica gel column chromatography is used for separating and purifying naturally existed alkyl resorcinol in the wheat bran, so that complexity and risks of chemical synthesis are greatly reduced, and operability is high.