The invention belongs to the field of leather processing, and specifically relates to a soft shoe upper leather processing method of sturgeon skin of Acipenser dabryanus and Chinese sturgeon. On the surface of sturgeon skin leather are distributed five columns of natural bone plates, but the requirement of shoe upper leather is hard to meet by a conventional softening technology. In allusion to the above problem, the technical problem during the sturgeon skin leather processing is solved emphatically. The processed sturgeon shoe upper leather is soft, plump and tear resistant, and is a high-quality material for manufacturing high-grade leather shoes.