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CN110007071A 一种CKAP4标记抗体-荧光微球复合物的制备方法及其应用 无效 - 驳回 转让

一种CKAP4标记抗体-荧光微球复合物的制备方法及其应用
摘要：
本发明涉及荧光免疫层析技术领域，具体涉及一种CKAP4标记抗体-荧光微球复合物的制备方法及其应用。一种CKAP4标记抗体-荧光微球复合物的制备方法，包括如下步骤：(1)用EDC振荡活化荧光微球；(2)使荧光微球与CKAP4标记抗体偶联；(3)加入5％-15％BSA溶液振荡封闭，离心，去上清；(4)加入保护液，超声，振荡偶联，离心，去上清；(5)加入5％-15％BSA溶液，振荡封闭，振荡结束后离心，去上清，沉淀用保护液吹散，即得所述CKAP4标记抗体-荧光微球复合物溶液。该CKAP4标记抗体-荧光微球复合物用于制备免疫荧光层析试纸条可实现CKAP4的快速检测，线性范围宽。
摘要（英）：

The invention relates to the technical field of fluorescence immunization chromatography, in particular to a preparation method and application of a CKAP4 labeled antibody-fluorescence microsphere compound and application of the preparation method. The preparation method of the CKAP4 labeled antibody-fluorescence microsphere compound comprises the following steps that (1) a fluorescence microsphere is oscillated and activated by EDC; (2) the fluorescent microsphere is coupled with a CKAP4 labeled antibody; (3) 5%-15% of a BSA solution is added to be oscillated and sealed, centrifugation is conducted, and supernatant is removed; (4) a protection solution is added, ultrasound, oscillation coupling, and centrifugation are conducted, and supernatant is removed; and (5) 5%-15% of a BSA solutionis added to be oscillated and sealed, centrifugation is conducted after oscillation is finished, supernatant is removed, precipitate is blown away through the protection solution, and a CKAP4 labeledantibody-fluorescence microsphere compound solution is obtained. The CKAP4 labeled antibody-fluorescence microsphere compound is used for preparing an immunization fluorescence chromatography test strip, quick detection of CKAP4 can be achieved, and the linearity range is wide.

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