The invention discloses a method capable of maintaining apitoxin activity, including A: subjecting wasps to quick-freezing and cryopreservation, B: long-distance transporting quick-frozen wasps, and C: thawing quick-frozen wasps, and acquiring the apitoxin. The main method of the invention is to quick-freeze fresh wasps and bees, and then extract and use active apitoxin. The use method of apitoxinobtained from quick-frozen wasps and bees solves the problems of the traditional apitherapy which needs to breed fresh wasps and bees, long-distance transportation of fresh wasps and bees and no fresh wasps and bees in winter. The activity of apitoxin in wasp/bee poison glands and the type of apitoxin are not changed by the quick-freezing method. The method preserves the activity of apitoxin, andis simple in operation and easy to grasp; there is no hidden danger of stress rejection which is common in wasp apitherapy after the apitoxin is used, so that the apitoxin can be easily accepted by the body, thus improving the use effect of apitoxin, and the method is convenient in transportation and good in popularization value.