The invention discloses a ludisia discolor tissue culture rapid propagation method. By means of explant selection and selection, inducing culture, enrichment culture, strong seedling rooting and transplantation, the ludisia discolor tissue culture rapid propagation method can obtain a large quantity of high-quality ludisia discolor seedlings. The ludisia discolor tissue culture rapid propagation method can achieve rapid culture and propagation of ludisia discolor and save adding of plant growth regulator during strong seedling rooting; the cultured ludisia discolor seedlings can be high in planting rate and quality, good in post-planting adaptability, and safe and environmentally friendly when applied as medicinal plants; by means of biological technology, the demands of commercialized production can be met within a short time, and technical support can be provided for scale production of ludisia discolor of enterprises. Meanwhile, ludisia discolor tissue culture rapid propagation method is good in sterilizing effect, rapid in culture and propagation and short in culture cycle which is around 198 days, and reduces contamination during tissue culture of the ludisia discolor, therebygreatly improving the planting rate and the working efficiency and saving resources.