The invention relates to the technical field of biological detection, in particular to an immune analysis detection kit for polypeptide related to IgA nephropathy. The kit comprises the following components: an enzyme label plate coated with a specific antibody of Pep16, a protein standard of Pep16, an enzyme-labeled mouse anti-Pep16 monoclonal antibody, 10x washing liquid, a color developing agent and a reaction termination liquid. The kit disclosed by the invention is stable, rapid, sensitive and accurate in detection, strong in specificity and high in sensitivity, and can quantitatively detect the content of the polypeptide Pep16 related to IgA nephropathy.