The invention discloses a graphene oxide-loaded column 5 aromatics lithium-sulfur battery separator, a preparation method and application thereof. According to the graphene oxide-loaded column 5 aromatics lithium-sulfur battery separator, aminated column 5 aromatics are loaded on a graphene oxide solution to form an aminated column 5 aromatics-loaded graphene oxide solution, which is then mixed with a certain amount of ethanol solution, the mixed solution is pumped dry on the separator Celgard 2400 of the lithium-sulfur battery by pumping filtration, and finally the material is obtained aftervacuum drying. The separator material has good electrochemical advantages and can effectively prevent polysulfide from flowing to the negative electrode of lithium-sulfur battery.