The invention discloses a fish and shrimp chili pepper sauce food comprising the following raw materials in parts by weight: 10-30% of a main ingredient, 10-30% of chili pepper, 40-60% of rapeseed oil, 8-12% of fermented soya beans, 1-2% of monosodium glutamate, 1-2% of edible salt, 0.5-1% of white granulated sugar and 0.5-1% of spices. The preparation method comprises the following steps: S1, stir-frying; S2, metering filling; S3, sterilization treatment; S4, cleaning and drying; S5, external packaging; and S6, inspection and delivery. The main ingredient and the chili pepper are treated carefully, so the sauce is clean and sanitary, and safe to eat; the chili pepper sauce prepared by mixing fishes or shrimps as the main ingredient with the chili pepper is not only delicious but also richin multi-vitamins, provides nutrition needed by a human body, has high nutritional value, has soft meat and is easy to digest by the human body. At the same time, the preparation method is scientificand effective, is simple in process, can better meet the needs of the market, and is suitable for large-scale industrial promotion.