The invention provides novel strain lactobacillus plantarum YL15 and application thereof in red wine flavored yogurt. Lactobacillus plantarum YL15 is separated and screened from acid horse milk collected from farmers and herdsmen in Urumqi, Xinjiang, the separated strain is utilized to prepare a fermentation preparation, the fermentation preparation is applied to the red wine flavored yogurt, theprepared red wine flavored yogurt has uniform color, is light pink, has stable traits, unique flavor and full taste and is a typical novel yogurt product, which has wide application value for the technical field of microbial strain application.