The invention provides an anti-plant virus epsilon-polylysine high-yield strain and a preparation process. The name of the high-yield strain is Streptomyces microflavus var. liaoningen Sis X, the preservation unit is the General Microbiology Center of the Chinese Microbiological Culture Collection and Management Committee, the preservation number is CGMCC No.15355, and the preservation date is February 12, 2018; the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City. The preparation process of anti-plant virus epsilon-polylysine comprises the following steps of strain propagation, anti-plant virus epsilon-polylysine generation by fermentation and finished product preparation process after fermentation. By adopting the anti-plant virus epsilon-polylysine high-yield strain and the preparation process, the epsilon-polylysine yield can be greatly improved, and the process has very important significance in industrial large-scale epsilon-polylysine production.