The invention provides a culture medium for obtaining megakaryocytes and platelets, a combined reagent and a kit, and a use thereof, and a method for obtaining the megakaryocytes and the platelets. The culture medium for obtaining the megakaryocytes and the platelets includes a basic culture medium, and a compound represented by the formula (1). The culture medium can induce erythroid cells to reprogram into megakaryocytes, and further generate the platelets. Without relying on transcription factors, a large number of megakaryocytes and platelets can be obtained, and broad application prospects are achieved.