The invention discloses a rice cytoplasm kinase encoding gene OsRLCK5 and application thereof. The nucleotide sequence of the gene is shown as SEQ ID No.1. The cytoplasm receptor kinase OsRLCK5 gene is connected with an over-expression vector through a genetic engineering means and then is transformed into rice, the expression of the gene in the transformed rice plant is improved, and the resistance of the rice plant to sheath blight can be improved; in addition, the expression of the homologous gene in other plants can be overexpressed, and the disease resistance of other plants can be enhanced. Furthermore, proteins encoded by the OsRLCK5 gene can be used as bait proteins for fishing out proteins interacted with the proteins in rice or other plants, and the regulation mode of the gene inthe process of resisting sheath blight is confirmed.