The invention discloses a titanium-based titanium oxide bipolar plate and a preparation method thereof; a micro-arc oxidation process is adopted; the method comprises the steps of firstly, oxidizing atitanium plate into a carbon-containing titanium dioxide ceramic layer, and then reducing the titanium dioxide into a titanium oxide (Ti4O7) ceramic layer with a conductive function through a carbonthermal reduction process. The process is simple and easy to operate, short in period, stable in process and high in repeatability. The method can be used for preparing the large-size titanium-based titanium oxide bipolar plate; the surface titanium oxide conductive ceramic layer is uniform in performance, and the conductivity can reach 700S/cm or above; and by taking 3M of H2SO4 as an electrolyte, an electrochemical linear polarization method is adopted, so that the bipolar plate with the corrosion rate of being less than 0.1[mu]A/cm2 can be obtained; and the preparation process has the characteristics of no toxic substance generation, environmental protection and high efficiency.