The invention discloses a method for promoting the solution compatibility of hyaluronic acid type substances and type II collagen and a composition containing the hyaluronic acid type substances and the type II collagen. By adding methotrexate polyglutamate, the condition that the hyaluronic acid type substances and the type II collagen form flocculent polyionic precipitates when meeting in a water solution is prevented. The problem that the hyaluronic acid type substances and collagen type substances can easily form the polyionic precipitates when meeting in water can be solved, so that a uniform and transparent water solution can be formed; the flocculent precipitates cannot occur after the long-period storage; the stability is high; the product quality is improved. Compared with the prior art, the method has the advantages that the structures of the hyaluronic acid type substances and the type II collagen cannot be damaged; the hyaluronic acid type substances, the type II collagen and the methotrexate polyglutamate are combined to achieve a very good moisture preserving effect; the application field is expanded; the safety is improved; very good application prospects are realized in the fields of food, cosmetics or medical instruments.