The invention discloses a method for setting up a tobacco black shank resistance identification disease nursery. The method comprises the steps that firstly, a flat land parcel suitable for tobacco planting is selected, and a variety likely to be infected with black shank is selected to be planted, and inoculated with black shank germs; at the later maturation stage, the stalk of a diseased planted in the field is crushed and evenly scattered in the land parcel of the disease nursery, and then ploughing and earthing are carried out; next year, the variety likely to be infected with the black shank and a moderate resisting variety are selected to be inoculated with the black shank germs and not to be inoculated with the black shank germs respectively, the disease-resistant grade of the inoculated variety and the variety which is not inoculated are identical, and the land parcel is used as the black shank disease nursery for identifying the black shank resistance of the tobacco variety;if the grades are different, the stalk of the diseased plant continues to be crushed and then evenly scattered to the land parcel of the disease nursery, ploughing and earthing are carried out, planting is carried out again, and identification is carried out until the grades are identical. According to the method, the environmental restriction factors that the time needed for setting up the diseases nursery is long, the land parcel selecting requirement is high, and many unstabilizing factors exit are overcome, and the method for setting up the tobacco black shank resistance identification disease nursery is high in fast setting-up accuracy and high in repeatability.