The invention belongs to the technical field of aquaculture, and discloses a running water fish culture tank, shrimp and crab serial circulating aquaculture system and method. Fishes are cultured in aplurality of parallel running water culture tanks arranged on a ridge, microporous aeration devices are arranged at the bottoms of the running water culture tanks, and fish screens are arranged at the two ends of each running water culture tank; an airlift-type aeration water push machine is installed on the upstream of the running water culture tanks, and a sewage collection pond is arranged onthe downstream of the running water culture tanks; a plurality of conical sewage collection hoppers are arranged at the bottom of the sewage collection pond; an artificial floating bed is placed at the tail end of the sewage collection pond; fishes are cultured and aquatic plants are planted in an artificial wetland purification area formed in the downstream of the sewage collection pond; the rearend of the sewage collection pond is communicated with a shrimp pond; a crab pond is adjacent to the shrimp pond, and aquatic plants grow in the shrimp pond and the crab pond; snails are put into a purified water reutilization area at the rear end of the crab pond. By means of the running water fish culture tank, shrimp and crab serial circulating aquaculture system and method, reutilization of water resources is realized, and the effect of zero emission is achieved; besides, several kinds of aquatic products are cultured in the system at the same time, and thus the economic benefit of culture is increased.