The invention relates to a laying hen feed containing a waste medium of cordyceps militaris sporophore and a preparation method thereof. The method comprises the following steps: (1) adding xanthan gum to water, controlling concentration of 0.2 to 0.4%, heating and dissolving the material, and cooling the material to obtain a granulating glue solution; (2) stirring the waste medium of cordyceps militaris sporophore and adding the material to the granulation glue solution, wherein the weight ratio of the waste medium to the granulation glue solution is 1:0.5-0.6, stirring the materials for 5-10minutes, performing granulation on a sieve, subsequently, drying the material in a boiling dryer at 60 to 70 DEG C to a moisture content of 12 to 14% to obtain waste medium particles of cordyceps militaris; (3) pulverizing corn, soybean meal, wheat bran and cotton meal to a particle size of 2 mm or less; and (4) uniformly mixing the materials in a blender according to the formula. The laying henfeed has the advantages that: (1) the feed particle size is uniform; (2) the laying hen feed has active ingredients such as cordyceps polysaccharide, cordycepin, and the like, the feed can increase the immunity of laying hens, reduces the dead rate of laying hens, and increases the laying rate of laying hens, and (3) the utilization of the waste medium is increased.