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发明公开
CN110093356A 编码非洲猪瘟病毒抗原的DNA序列、由其编码的抗原的组合物及其在免疫学检测中的应用 有权 转让

编码非洲猪瘟病毒抗原的DNA序列、由其编码的抗原的组合物及其在免疫学检测中的应用
摘要：
本发明提供了一种编码非洲猪瘟病毒抗原的DNA序列、包含上述DNA序列的载体、包含上述载体的表达系统，由上述DNA序列编码的抗原的组合物、以及上述抗原组合物在检测非洲猪瘟病毒的免疫学检测方法中的应用。本发明通过消除稀有密码子等密码子优化的方法对编码非洲猪瘟病毒的主要膜蛋白和衣壳蛋白中的CP204L、PK205R、PB602L、CP530R、E183L、以及PB646L的DNA序列进行了优化，并且发现上述经密码子优化的核苷酸序列可以在合适的表达系统中高效地表达上述主要膜蛋白和衣壳蛋白，并且由此建立的免疫学检测方法可以快速、简便、大规模地检测非洲猪瘟病毒。
摘要（英）：

The invention provides a DNA sequence encoding African swine fever virus antigen, a composition of the antigen encoded thereby and use thereof in immunological detection. and also provides an application of the composition of the antigen encoded by the above DNA sequence, and the above antigen composition for an immunological detection method for detecting the African swine fever virus. A codon optimization method capable of eliminating rare codons is used for optimizing the DNA sequences of CP204L, PK205R, PB602L, CP530R, E183L, and PB646L in the major membrane proteins and capsid proteins encoding the African swine fever virus, the above-described codon-optimized nucleotide sequence can efficiently express the above-mentioned main membrane proteins and capsid proteins in a suitable expression system, and an established immunological detection method can detect Africa swine fever virus quickly and simply on a large scale.

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