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发明公开
CN110093432A 一种用于区分结核杆菌和BCG疫苗的sRNA标志物及其用途 有权

一种用于区分结核杆菌和BCG疫苗的sRNA标志物及其用途
摘要：
本发明公开了一种用于区分结核杆菌和BCG疫苗的sRNA标志物及其用途。为了解决结核杆菌感染和BCG疫苗接种难以区分的问题，本发明发明人首先通过细菌RNA测序，检测和筛选结核分枝杆菌中的sRNA种类，按照本发明中筛选到的目标sRNA序列设计了能够检测结核杆菌sRNA的特异性引物、试剂盒，并建立了相应的实时荧光定量PCR检测方法。从细菌菌体中提取游离sRNA，合成cDNA后，用本发明的实时荧光定量PCR法对其含量进行检测，实验证明该方法可用于区别结核杆菌和BCG疫苗，并且具有敏感性高、特异性好、成本低、操作方便等优点，有望进一步满足临床以及实验室对于结核杆菌和BCG疫苗快速诊断的需要。
摘要（英）：

The invention discloses an sRNA marker for distinguishing mycobacterium tuberculosis and a BCG vaccine and a use thereof. For solving the problem that mycobacterium tuberculosis infection and BCG vaccination are difficult to distinguish, the type of sRNA in mycobacterium tuberculosis is detected and screened by bacterial RNA sequencing firstly, a specific primer and kit capable of detecting the sRNA of mycobacterium tuberculosis are designed according to the screened target sRNA sequence, and a corresponding real-time fluorescence quantitative PCR detection method is established. After free sRNA is extracted from bacterial bodies and cDNA is synthesized, the content of sRNA is detected by the real-time fluorescent quantitative PCR method. The experiment proves that the method can be used for distinguishing the mycobacterium tuberculosis and the BCG vaccine, has the advantages of high sensitivity, good specificity, low cost, convenience in operation and the like, and is hopeful to further meet the clinical and laboratory needs for rapid diagnosis of the mycobacterium tuberculosis and the BCG vaccine.

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