The invention discloses an anti-seismic design method for overhead pipeline laying in an oil and gas transmission tunnel. The method comprises the steps that the pipeline allowable span is preliminarily calculated and determined; pipeline anti-seismic checking is carried out, and a pipeline calculation span is determined; the span is calculated according to the determined pipeline, and the seismicforce of the pipeline concrete buttress is calculated; and then the arrangement structure of the concrete buttresses in the tunnel and the connection mode of the concrete buttresses and the pipelinesare determined. The method has the beneficial effects that the pipeline allowable span and the pipeline concrete buttress structure seismic force are determined through calculation, the pipeline concrete buttress structure is designed according to the calculation result, the position relation between the gravity center of the pipeline and the concrete buttress is changed, and the shear capacity of the concrete structure is fully utilized; meanwhile, the pipeline anchoring pier is designed to bear the axial seismic force of the pipeline; the structures and material characteristics of the pipeline, the pipeline concrete buttresses, the pipeline anchoring piers and the pipeline connecting pieces are fully utilized, the anti-seismic structure and measures are optimized and improved, and the anti-seismic capacity of the overhead pipeline system in the tunnel is improved.