The invention discloses an online intelligent diagnosis platform based on big data and an operation method and a storage medium thereof. The operation method comprises the steps that an eye ground image of a to-be-detected object is received; the eye ground image of the to-be-detected object is matched with standard eye ground images corresponding to all the stages of the disease; according to a matching result, the disease stage of the to-be-detected object is judged; a convolution kernel is selected in the disease stage of the to-be-detected object, and the selected convolution kernel is adopted for conducting convolutional neural network algorithm processing on the eye ground image of the to-be-detected object so as to extract pathology characteristic information; the pathology characteristic information is matched with data corresponding to the disease stage of the to-be-detected object in a database of a cloud platform so as to obtain intelligent diagnosis information. According to the technical scheme, the pressure of an ophthalmologist can be relieved, the diagnosis efficiency and accuracy can be improved, the calculation amount of the cloud platform is reduced, and the operation efficiency is improved.