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发明公开
CN110106272A 一种四倍体长穗偃麦草3E染色体分子标记及其应用 有权

一种四倍体长穗偃麦草3E染色体分子标记及其应用
摘要：
本发明公开了一种四倍体长穗偃麦草3E染色体分子标记及其应用。该分子标记是以四倍体长穗偃麦草的基因组DNA为模板，采用引物对1～20中的任一对扩增得到的。通过对二倍体、四倍体长穗偃麦草、硬粒小麦-四倍体长穗偃麦草双二倍体和小麦-四倍体长穗偃麦草1E-7E染色体代换系进行测序，获得四倍体长穗偃麦草3E染色体特异序列，开发了3E染色体的特异分子标记，并在小麦及其近缘属物种中进行了验证。该特异分子标记不仅可用于四倍体长穗偃麦草3E染色体的快速检测，而且可以准确地鉴别四倍体长穗偃麦草与二倍体、十倍体长穗偃麦草及其他近缘属物种染色体，为小麦族系统进化、种质资源鉴定和分子标记辅助选择育种提供了重要途径。
摘要（英）：

The invention discloses a tetraploid elytrigia elongata 3E chromosome molecular marker and application thereof. According to the molecular marker, with tetraploid elytrigia elongata genome DNA as a template, one of 1-20 primer pairs is adopted for amplification, and the molecular marker is obtained. By sequencing 1E-7E chromosome substitution lines of diploid and tetraploid elytrigia elongata, durum wheat-tetraploid elytrigia elongata amphidiploid and wheat-tetraploid elytrigia elongata, a specific sequence of a tetraploid elytrigia elongata 3E chromosome is obtained, the specific molecular marker of the 3E chromosome is developed, and verification is conducted for wheat and related genera species of the wheat. The molecular marker can be applied to quick detection of the tetraploid elytrigia elongata 3E chromosome and can accurately identify the chromosomes of the tetraploid elytrigia elongata, the diploid elytrigia elongata, the decaploid elytrigia elongata and other related genera species, and an important way is provided for phyletic evolution of triticeae, germplasm resource identification and auxiliary selection breeding of the molecular marker.

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