The invention discloses an agricultural mulching film one-piece recycling and rolling mechanism which comprises a film roller, a supporting frame, a square connection rod, a connection barrel, a connection shaft and a wrench B. The film roller comprises a roller, a transmission shaft, a roll shaft, a roller supporting frame, a connection rod, an ejector pin, a spring and a wrench A, the supportingframe is composed of a bottom plate, a left supporting plate, a right supporting plate and a middle plate, the left supporting plate, the right supporting plate and the middle plate are vertically fixed on the bottom plate, and the middle plate is positioned between the left and right supporting plates; the connection barrel is mounted in a bearing on the left supporting plate, the square connection rod penetrates a square through hole at the center of the connection barrel, and the right end of the square connection rod is connected in a square hole at the left end of the transmission shaft;the connection shaft is mounted in a bearing on the right supporting plate, the right end of the transmission shaft is connected with the left end of the connection shaft through a coupler and a flatkey, and the right end of the connection shaft is connected with a motor output shaft. The mechanism is supportive of quick demounting of a mulching film completely, convenient to operate and conducive to one-piece recycling and reutilizing of the mulching film.