The invention discloses an atlantoaxial vertebrae fixing plate with guiding holes and a using method of the atlantoaxial vertebrae fixing plate. The atlantoaxial vertebrae fixing plate comprises a plate body, wherein fixing plates are arranged at four corners of the plate body; bone grafting grooves are formed in four edges of the plate body; a plurality of guiding holes are formed in each fixingplate, and the guiding holes are in different angles; guiding threads are formed in the inner surface of each guiding hole; an axial lead of each guiding thread is coincided with that of the corresponding guiding hole; a plurality of positioning holes are formed in each fixing plate; and the positioning holes are in different angles. According to the atlantoaxial vertebrae fixing plate with the guiding holes and the using method of the atlantoaxial vertebrae fixing plate disclosed by the invention, the individual atlantoaxial vertebrae fixing plate is customized for a patient, so that the angles, the numbers and the positions of the guiding holes and the positioning holes are more reasonable; the designing directions of the guiding holes and the positioning holes are consistent with screwplacing directions, operation is kept away from important tissue of the patient, the situation that the fixing plate is grafted relying on the experience of operators is avoided, the situation that the atlantoaxial vertebrae screws are wrongly placed is avoided, and the treatment effect is strengthened.