The present invention relates to a quadruple fluorescence quantitative detection kit for simultaneously detecting four human coronaviruses, and belongs to the technical field of human coronavirus detection. The human coronaviruses are specifically MERS-CoV/HCoV-NL63/HCoV-OC43/HCoV-HKUI; the detection kit specifically comprises four groups of specific primer pairs and probes, a positive quality control product and a negative quality control product; each group of the specific primer pairs and probes all comprises a pair of specific primers and a probe sequence; the positive quality control product is specifically a artificially synthetic target sequence; and the negative quality control product is deionized water or sterile water. The kit can simultaneously detect the four human coronaviruses by a single tube, is strong in detection specificity, high in detection sensitivity and fast in detection time, and avoids problems that one-by-one detection is long in time-consuming, high in detection cost, easy for cross-contamination, etc. No similar related product is reported on the market at present. The kit has broad application prospects in disease detection, prevention and control ofinfectious diseases, clinical treatment guidance, etc.