The invention discloses a preparation method of car seat leather with excellent fold resistance, wear resistance and aging resistance. The preparation method comprises steps as follows: A, bonding layer slurry, middle layer slurry and skin layer slurry are prepared, and base cloth and release paper are selected; B, the release paper is coated with the prepared skin layer slurry, and a skin layer is formed; C, the skin layer is coated with the prepared middle layer slurry, and a middle layer is formed; D, the middle layer is coated with the prepared bonding layer slurry; E, then the release paper is attached to the microfiber base cloth, drying and ripening are performed, the release paper is stripped, and the car seat leather is obtained; F, surface printing slurry is prepared; G, the other surface of the skin layer of the car seat leather is coated with the prepared surface printing slurry, drying and cooling are performed, and the car seat leather with excellent fold resistance, wearresistance and aging resistance is obtained. The car seat leather which is prepared with the method and has excellent fold resistance, wear resistance and aging resistance conforms to general GMW14122 standard.