The invention provides a bicycle tube battery anti-dropping mechanism for an electric bicycle and a using method thereof. The bicycle tube battery anti-dropping mechanism comprises a bicycle tube, wherein an open slot is formed in the lower portion of the bicycle tube; a battery base which is matched with the open slot is fixed in the open slot; a telescopic lock tongue is arranged on the inner side surface of the open slot; the end part of the battery base is provided with a lock slot which is matched with the lock tongue to achieve pre-supporting effect for preventing battery base dropping;and the surface, which is matched with the front end of the lock tongue, of the upper surface in the lock slot is an inclined surface. The bicycle tube battery anti-dropping mechanism is reasonable indesign, is simple in structure, can effectively prevent the battery base from directly dropping during unlocking to cause battery base damages, facilitates clamping, and has practicability and innovation.