The invention provides a sealing and connecting mold of a middle glass frame and a rear cover plate of a cell phone and a use method of the sealing and connecting mold. The sealing and connecting moldcomprises a first mold and a second mold in corresponding match, wherein rectangular grooves which are in corresponding match with the rear cover plate of the cell phone are formed in the first moldand the second mold; columns which are in corresponding match with the rear cover plate of the cell phone are formed in the bottoms of the grooves; and width pressing plates and length pressing platesin corresponding match are connected with the rectangular grooves in a clamped manner. The rear cover plate of the cell phone is put into the rectangular grooves, the middle glass frame is placed later, the middle glass frame is limited trough the width pressing plates and the length pressing plates, later the rectangular grooves are put into a high-temperature furnace for high-temperature heat preservation, and a combined body of the middle glass frame and the rear cover plate of the cell phone can be manufactured after demolding. The sealing and connecting mold is simple in structure, convenient to assemble, simple in processing procedure and low in manufacturing cost, and in addition, the produced combined body of the middle glass frame and the rear cover plate of the cell phone has the advantages of being good in appearance, good in hand feeling, free of signal interruption, and the like.