The invention discloses an auxiliary tool for assembling glass beads and an assembling method. The auxiliary tool comprises a master carrier and a slave carrier for covering the master carrier. The master carrier is provided with a side-by-side installing passage corresponding to the mounting hole of a connector. The slave carrier is provided with a stopping passage corresponding to the side-by-side installing passage. A hole passage is formed by the side-by-side installing passage and the stopping passage. The master carrier is provided with a first socket. The slave carrier is provided witha second socket. The first socket and the second socket form an insertion slot to be connected to the assembling hole of the connector. The auxiliary tool solves the problem that visual fatigue is likely to occur in the conventional glass bead assembly and the conventional glass bead assembly is low in efficiency.