The invention discloses a traditional Chinese medicine composition for preventing and treating drug-induced liver injury, and a preparation method and application thereof, and relates to the technicalfield of medicines for preventing and treating liver diseases. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 9 to 30 parts of radix astragali, 6 to 15 parts of herba artemisiae scopariae, 10 to 15 parts of radix salviae miltiorrhizae, 6 to 10 parts of vinegar-processed rhizoma cyperi, 6 to 15 parts of fried radix paeoniae alba, 15 to30 parts of herba hyperici japonici, 5 to 9 parts of calyx seu fructus physalis, 3 to 10 parts of radix bupleuri, 2 to 6 parts of fructus schisandrae, 6 to 12 parts of fried rhizoma atractylodis macrocephalae, 3 to 10 parts of radix scutellariae, 6 to 12 parts of fructus broussonetiae, and 2 to 10 parts of raw licorice roots. The traditional Chinese medicine composition of the invention is safe,reliable and effective, has no toxic and side effects, has good preventive and therapeutic effects on drug-induced liver injury at low prevention cost, and brings good news for patients with drug-induced liver injury.