The invention relates to the technical field of bioengineering, and particularly relates to a method and a medium for amplifying bone-marrow mesenchymal stem cells and application. The method for amplifying bone-marrow mesenchymal stem cells comprises the steps of treating the bone-marrow mesenchymal stem cells with bFGF and VEGF to amplify the bone-marrow mesenchymal stem cells. The bFGF and VEGFare added to the medium. The bone-marrow mesenchymal stem cells cultured by the method provided by the invention can increase the proliferation ability of the cells, and maintain the biological characteristics and functions of the cells, and also can increase the expression rate of the cell surface molecule CD105, thereby being useful for transplant treatment of acute myocardial infarction, chronic myocardial infarction, and ischemic heart disease caused by cardiac failure.