The invention relates to a tobacco stem stick form defining method. The defining method is composed of a stem stick appearance form statistical step, a sensory quality evaluation step and a combustiontest step. According to the method disclosed by the invention, the appearance morphology of the stem sticks is classified and counted, and the research on the burning and smoking sensory influence ofthe cigarettes according to the stems with different forms is studied, and definition standard of the stem sticks in the cigarettes is obtained, so that the accurate basis can be provided for controlling the content of the stem sticks in the cigarette cut tobacco and evaluating the stem stick removing effect of a cigarette making machine.