The invention discloses a surface-charge-based pipetting gun. The pipetting gun comprises a gun body, wherein a plurality of support rods are arranged under the gun body; connecting rods are arrangedon the lower ends of every two adjacent support rods; a base is arranged between the connecting rods; a surface coating is arranged on the base; the surface coating is provided with a hydrophobic molecular layer by vapor deposition; the surface coating and the hydrophobic molecular layer form a super-amphiphobic surface on the base; the base and the surface coating are both made of dielectric materials; a circular limiting plate is arranged above the gun body; the gun body, the support rods, the connecting rods and the limiting plate are integrally formed, and are not electrically conductive;an electrically conductive sliding bar is movably arranged in the gun body and the limiting plate; the lower end of the sliding bar is provided with a sheet conductor; the upper end of the sliding baris provided with a push plate. According to the surface-charge-based pipetting gun, a spearhead is replaced by the super-amphiphobic surface; when pipetting is performed, droplets impact the super-amphiphobic surface, an electric charge is generated on the surface, and then the super-amphiphobic surface after charging has high adhesion to the droplets, thereby realizing the grab and movement of the droplets.