The invention provides a stable base structure with adjustable trundles and an adjusting method of the stable base structure, and belongs to the field of machine tool components. The stable base structure solves the problem that lifting adjustments of the universal trundle are troublesome. The stable base structure with the adjustable trundles comprises an upper supporting plate and a lower supporting plate, and further comprises four wheel seats; an upper supporting block and a lower supporting block are arranged in the wheel seat; the universal trundle is fixed to the bottom end of the lowersupporting block. Two supporting seats are fixed between the upper supporting plate and the lower supporting plate; supporting holes are formed in the two supporting seats; the stable base structurefurther comprises an adjusting rod, an inner adjusting pipe and an outer adjusting pipe; the outer adjusting pipe penetrates through the supporting holes and is fixedly provided with a check ring; thestable base structure further comprises an operating rod and an operating seat arranged on the operating rod in a sleeving mode; the operating seat and a front end of the inner adjusting pipe are fixed, and a one-way forward rotating mechanism and a one-way reverse rotating mechanism are arranged between the adjusting rod and the inner adjusting pipe; a connecting seat is further screwed on the outer adjusting pipe; the four upper supporting blocks and the connecting seat are connected through connecting pieces to form a linkage structure. The stable base structure can drive the universal trundles to synchronously ascend and descend.