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发明公开
CN110313404A 一种紫薇组织培养再生体系的构建方法 无效 - 驳回

一种紫薇组织培养再生体系的构建方法
摘要：
本发明属于紫薇组织培养技术领域，公开了一种紫薇组织培养再生体系的构建方法，包括收集种子、选择优良单株、扦插、嫁接组织培养，并进行叶芽诱导、愈伤诱导；其中叶芽诱导包括选取叶芽、丛生芽、生根、移栽；愈伤诱导包括选取叶和径、愈伤、丛生芽、生根、移栽；组织培养或采用体胚诱导进行。本发明解决了因为紫薇杂合度高，种子播苗形状分离变异大，紫薇的种植成本增高的问题；本发明通过对外植体、诱导培养基、继代培养基及生根培养基的选取，能够使得萌发率达到80-90％，增殖率达到20倍，生根率达到90％，能够高效的得到紫薇无菌苗。
摘要（英）：

The invention belongs to the technical field of crape myrtle tissue culture, and discloses a construction method of a crape myrtle tissue culture regeneration system. The construction method comprisesthe steps of seed collecting, selection of excellent single plants, cuttage, grafting for tissue culture, leafbud induction and callus induction, wherein leafbud induction involves selection of leafbuds and cluster buds, rooting and transplanting, and callus induction involves selection of leaves and stems, calluses and clusters, rooting, and transplanting. For tissue culture, somatic embryos areadopted for induction. The construction method solves the problems that the heterozygosity of crape myrtle is high, the seed seedlings have large variations of character segregation, and the plantingcost of crape myrtle is increased. According to the construction method, by selecting explants, an induction culture medium, a subculture medium and a rooting culture medium, the germination rate canreach 80-90%, the multiplication rate reaches 20 times, the rooting rate reaches 90%, and crape myrtle aseptic seedlings can be efficiently obtained.

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