The invention discloses a production method of an HE-stained slice. The production method comprises the steps that a white slice is roasted, dewaxed, hydrated, HE-stained and packaged, wherein the step of treating the white slice, obtained after hydrating treatment, with a metal ion complexant solution is further included between hydrating and HE-staining. According to the production method, whenthe HE-stained slice is produced, treatment with the metal ion complexant solution is added between the hydrating step and the HE-staining step, and a metal ion complexant can effectively reduce or eliminate tissue cracks, so that tissue morphology is clearer; and cell pyknosis and overlapping can be effectively reduced, so that the accuracy and reliability of tissue and cell interpretation are greatly enhanced.