The invention discloses a radioactive nuclide enrichment method and device, and a system with same. The method comprises steps of collecting to-be-tested liquid, and controlling flow of the to-be-tested liquid; in the process of concentration and enrichment, heating and stirring to evaporate the to-be-tested liquid according to a preset target temperature difference and a target boiling heat transfer requirement, so as to enable evaporation speed to meet preset speed; after cleaning, collecting concentrated solution after enrichment of the radioactive nuclide, and obtaining a radioactive nuclide enrichment result of the to-be-tested liquid according to the concentrated solution. In the prior art, enrichment takes long time and is slow in speed, enrichment relies on technical level of an operator, and data of the same batch of processing is less consistent. The method solves the above technical problems. The vacuum, low-temperature enrichment method shortens enrichment time greatly, enables automatic operation, makes enriched samples more consistent, and is easy to implement.