The invention provides powder for settling pain and promoting tissue regeneration. The powder is prepared from, by weight, 7-10% of realgar, 7-10% of fossil fragments, 7-10% of calomel, 7-10% of myrrh, 7-10% of olibanum, 7-10% of catechu, 7-10% of the root of common peony, 7-10% of cuttlebone and 30-44% of borneol. The powder for settling the pain and promoting tissue regeneration can settle the pain, promote tissue regeneration, kill germs and prevent infection and aggravation of a wound, further can promote quick healing of the wound, and can remove scars generated by healing of the wound.