The invention discloses a standby automatic switching misoperation prevention method for a 110kV single bus fault and is applied to a 110kV single bus primary main wiring system network. The method comprises steps that the first condition, 10kV bus composite voltage blocking is open; the second condition, a 110kV bus differential current reaches a set value; when the first and second conditions are satisfied, a 110 kV standby power supply is automatically turned on; otherwise, the 110kV standby power supply is automatically put into a device exit. The method is advantaged in that in the case that a 110kV substation is not additionally equipped with a 110kV bus differential protection device, by adding the differential flow operation logic and the locking standby automatic switching execution logic, locking standby automatic switching closing operation can be correctly performed under the condition of the 110kV single bus fault, while economic cost is effectively reduced, the workload is reduced, the risk of 110kV standby automatic switching closing and the bus fault is further avoided, and the method is of great significance to table operation of a power system.