The invention relates to the technical field of mineral flotation, and discloses a spodumene flotation collector and a preparation method thereof and a spodumene flotation method. The spodumene flotation collector is prepared from the following components: 10-30 parts of dodecyl dimethyl tertiary amine, 150-450 parts of vegetable oleic acid and 25-75 parts of sodium hydroxide. The preparation method of the spodumene flotation collector comprises the following steps that the vegetable oleic acid and the dodecyl dimethyl tertiary amine are mixed evenly to obtain a mixed material; and the mixed material is mixed with the sodium hydroxide, heated and stirred until the reaction is complete to obtain the spodumene flotation collector. The invention further provides a spodumene flotation method by adopting the spodumene flotation collector. By using the spodumene flotation collector, spodumene can be subjected to flotation under normal temperature, the collection capacity can be effectively exerted under low temperature, and meanwhile, the spodumene flotation collector can be applied to low-grade spodumene ore, and spodumene concentrate with high grade and recovery rate can be obtained.