The invention discloses a display base plate, a manufacturing method of the display base plate and a display panel. The display base plate comprises a base plate and a plurality of quantum dot color films, wherein the plurality of quantum dot color films are positioned on the base plate and are in array distribution; the quantum dot color films are configured to at least give out light in one color; each quantum dot color film comprises a plurality of regions in dense arrangement; the refractive indexes of the adjacent regions are different; and each region comprises quantum dots in uniform distribution and diffusion particles. Each quantum dot color film in the display base plate is divided into a plurality of regions, and the refractive indexes of the adjacent regions are different, so that the wide-angle emergent light is reduced; and non-emergent laser can be utilized again by quantum dots, so that the luminous efficiency and the brightness corresponding to the center angle of thequantum dot color films are improved.