The invention discloses a probiotics extracted biological fiber mask and a preparation method thereof, and belongs to the technical field of facial mask preparation. According to the probiotics extracted biological fiber facial mask, facial mask essence specifically comprises the following components: aloes, pearl powder, skimmed milk powder, glucose, soluble starch, ethyl maltol, sodium alginate,food-grade sodium benzoate, protease, lipase, bacterial powder, egg white and honey. The preparation method specifically comprises the following steps: culturing probiotics; preparing aloe juice extract; preparing facial mask substrate solution; carrying out facial mask fermentation; adding aldehydo-D-glucuronic acid; processing the facial mask; and carrying out quality testing, bagging and warehousing. According to the invention, the aloe extract and the facial mask substrate solution are added into a lactic acid bacteria culture dish, and the biological fiber facial mask, prepared by addingthe licorice root powder and the potato extract powder in the preparation process, is strong in skin-friendly ability, high in mildness, high in antibacterial and anti-inflammatory properties and high in essence absorption rate in use.