The invention discloses a clathration method of essential oil. The clathration method of the essential oil comprises the steps that an ethanol solution of the essential oil is added into a water solution added with beta-cyclodextrin for stirring clathration to obtain clathration liquid; and a carbon nano tube is added into the clathration liquid, first ultrasonic treatment is carried out, then constant-temperature vibration, cold storage, filtration and freeze drying are carried out to obtain an essential oil clathration compound. The clathration method of the essential oil has high clathration rate, and the obtained essential oil clathration compound has good heat stability and long-term storage stability.