The invention discloses an internal and external network isolation penetration design method. The method comprises the following steps: S1, queuing a large number of load requests by utilizing an M/M/1/K/infinity/FCFS queuing idea; S2, introducing an extended thread pool on the basis of the basic thread pool, transmitting configuration parameters and control messages through a pool management module to obtain system performance indexes, and creating a new thread and a blocked extended thread according to a dynamic optimization strategy; S3, queuing a system; S4, performing a thread pool dynamic management mechanism; S5, giving a thread scheduling algorithm based on the M/M/1/K/infinity/FCFS queuing model and the two dynamic adjustment processes; and S6, designing a boundary security protection architecture for internal and external network information interaction. According to the method, by effectively adjusting the scale of the thread pool, the performance guarantee capability of theserver applying the method is improved, data in the penetration process are monitored and protected, and the safety and stability of the internal and external network environment are maintained.