The invention discloses a method for manufacturing tobacco sheets by using tobacco stalks. The method comprises the following steps: step (1), cutting dried tobacco stalk raw materials to form flat tobacco stalk segments; step (2), soaking the flat tobacco stalk segments in water, until the flat tobacco stalk segments are completely saturated; step (3), after the flat tobacco stalk segments are soaked, performing an enzymatic hydrolysis reaction by using basidiomycetes to form tobacco stalk slurry; step (4), stirring and pulping the tobacco stalk slurry to form coarse fiber bundles; step (5),performing extraction separation on the coarse fiber bundles, and performing concentration on the separated solvend to form a concentrated solution, and performing pulping treatment on the separated solid slurry; step (6) preparing slurry by using the extracted coarse fiber yarns; step (7), performing papermaking on the slurry to obtain tobacco stalk sheets; and step (8) performing drying treatment on the wet tobacco stalk sheets to obtain tobacco sheets. By adopting the method disclosed by the invention, the waste tobacco stalks are fully utilized, the cost of manufacturing the tobacco sheetsis reduced, the waste is turned into treasures, and the problem that a large number of germs are parasitic in the tobacco stalks, thereby easily spreading diseases, causing waste of resources and pollution of tobacco fields, is also solved.